The results of the pathological and parasitological examination indicated that it was caused by infection with a parasite of the Digenea group, which is the same group as the fish fluke, and causes inflammation of the tissue under the skin, including the formation of a membrane around the parasite larvae embedded in the fish flesh. It was also confirmed that the abnormal symptoms were not caused by heavy metal contamination or chemical residues in the Mekong River in any way, and it was not a severe contagious disease in fish caused by a virus.
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